Plpro 3

plpro 3

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In addition to this proteolytic activity, PLpro reverses cellular ISGylation and ubiquitination processes, and may deubiquinate some host cell. MPro assays were performed using the same assay buffer as for PLpro, with 10�M peptide and between 5 and nM enzyme. Caspase Internal. PLpro-IN-3 (compound 26R) is a potent Papain-like protease (PLpro) inhibitor with a IC50 of ?M. PLpro-IN-3 exhibits antiviral activity against SARS-CoV
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The results suggest that procyanidin may be more potent in inhibiting the deubiquitinating activity of SARS-CoV-2 PLpro compared to other flavonoids and lopinavir the reference compound. These collated findings will accelerate further structure-based drug design efforts targeting PLpro, with the ultimate goal of identifying high-affinity inhibitors of clinical value for SARS-CoV Here, we utilized substrate phage display coupled with protein network analysis to identify human proteome substrates of severe acute respiratory syndrome coronavirus 2 SARS-CoV-2 viral proteases, including papain-like protease PLpro and 3C-like protease 3CLpro.

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